Increased uncoupling protein-2 and -3 gene expressions in skeletal muscle of STZ-induced diabetic rats
作者： Haruaki Kageyama , Asako Suga , Misato Kashiba , Jun Oka , Toshimasa Osaka
DOI: 10.1016/S0014-5793(98)01506-3
关键词: Thermogenesis 、 Gastrocnemius muscle 、 Skeletal muscle 、 Endocrinology 、 Insulin 、 Uncoupling protein 、 Internal medicine 、 Biology 、 Brown adipose tissue 、 Streptozotocin 、 White adipose tissue
摘要: Streptozotocin (STZ)-induced diabetic animals are vulnerable to cold stress. Uncoupling proteins (UCPs) play an important role in regulating thermogenesis. We investigated the gene expressions of UCPs brown adipose tissue (BAT), white (WAT), liver and gastrocnemius muscle STZ-diabetic rats using Northern blot. UCP-1, -2 -3 mRNA BAT were all remarkably lower than those control rats. Both UCP-2 substantially elevated insulin treatment restored UCP normal levels. These results suggest that rats, overexpression UCP-3 skeletal provides a defense against hypothermogenesis caused by decreased BAT.
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sci-hub.se 参考文章(25)
M A Glotzer, U Stadler, L P Kozak, A Jacobsson, Mitochondrial uncoupling protein from mouse brown fat: molecular cloning, genetic mapping, and mRNA expression Journal of Biological Chemistry. ,vol. 260, pp. 16250- 16254 ,(1985) , 10.1016/S0021-9258(17)36228-2
F Bouillaud, J Weissenbach, D Ricquier, Complete cDNA-derived amino acid sequence of rat brown fat uncoupling protein. Journal of Biological Chemistry. ,vol. 261, pp. 1487- 1490 ,(1986) , 10.1016/S0021-9258(17)35962-8
Jean Himms‐Hagen, Brown adipose tissue thermogenesis: interdisciplinary studies The FASEB Journal. ,vol. 4, pp. 2890- 2898 ,(1990) , 10.1096/FASEBJ.4.11.2199286
D. Ricquier, L. Casteilla, F. Bouillaud, Molecular studies of the uncoupling protein. The FASEB Journal. ,vol. 5, pp. 2237- 2242 ,(1991) , 10.1096/FASEBJ.5.9.1860614
D G Nicholls, R M Locke, Thermogenic mechanisms in brown fat. Physiological Reviews. ,vol. 64, pp. 1- 64 ,(1984) , 10.1152/PHYSREV.1984.64.1.1
J. Seydoux, E.R. Trimble, F. Bouillaud, F. Assimacopoulos-Jeannet, S. Bas, D. Ricquier, J.P. Giacobino, L. Girardier, Modulation of β-oxidation and proton conductance pathway of brown adipose tissue in hypo- and hyperinsulinemic states FEBS Letters. ,vol. 166, pp. 141- 145 ,(1984) , 10.1016/0014-5793(84)80060-5
Alain Géloën, Paul Trayhurn, Regulation of the level of uncoupling protein in brown adipose tissue by insulin requires the mediation of the sympathetic nervous system FEBS Letters. ,vol. 267, pp. 265- 267 ,(1990) , 10.1016/0014-5793(90)80941-B
A. Krook, J. Digby, S. O'Rahilly, J. R. Zierath, H. Wallberg-Henriksson, Uncoupling protein 3 is reduced in skeletal muscle of NIDDM patients. Diabetes. ,vol. 47, pp. 1528- 1531 ,(1998) , 10.2337/DIABETES.47.9.1528
A. R. Scott, T. Bennett, I. A. Macdonald, Diabetes mellitus and thermoregulation Canadian Journal of Physiology and Pharmacology. ,vol. 65, pp. 1365- 1376 ,(1987) , 10.1139/Y87-215
Nobuyo Tsuboyama-Kasaoka, Nobuyo Tsunoda, Kayo Maruyama, Mayumi Takahashi, Hyounju Kim, Shinji Ikemoto, Osamu Ezaki, Up-Regulation of Uncoupling Protein 3 (UCP3) mRNA by Exercise Training and Down-Regulation of UCP3 by Denervation in Skeletal Muscles Biochemical and Biophysical Research Communications. ,vol. 247, pp. 498- 503 ,(1998) , 10.1006/BBRC.1998.8818