Mutations inCaenorhabditis briggsaeidentify new genes important for limiting the response to EGF signaling during vulval development
作者： Devika Sharanya Devika Sharanya , CJ Fillis , Kim JaeYoung Kim JaeYoung , EM , Jr Zitnik
DOI: 10.1111/EDE.12105
关键词: Gene mapping 、 Cloning 、 Gene 、 Biology 、 Signal transduction 、 Genetics 、 Wild type 、 Wnt signaling pathway 、 Phenotype 、 Caenorhabditis briggsae
摘要: SUMMARY Studies of vulval development in the nematode C. elegans have identified many genes that are involved cell division and differentiation processes. Some these encode components conserved signal transduction pathways mediated by EGF, Notch, Wnt. To understand how developmental mechanisms change during evolution, we doing a comparative analysis vulva formation briggsae, species is closely related to elegans. Here, report 14 mutations 7 Multivulva (Muv) briggsae inhibit inappropriate precursors. We developed new efficient cost-effective gene mapping method localize Muv small genetic intervals on chromosomes, thus facilitating cloning functional studies. demonstrate utility our determining molecular identities three include orthologs Cel-lin-1 (ETS) Cel-lin-31 (Winged-Helix) EGF-Ras pathway Cel-pry-1 (Axin), Wnt pathway. The remaining four reside regions lack known genes. Inhibitor studies phenotype all dependent activity EGF kinase, MEK. One these, Cbr-lin(gu167), shows modest increase expression Cbr-lin-3/EGF compared wild type. These results argue while Cbr-lin(gu167) may act upstream Cbr-lin-3/EGF, other influence downstream or parallel Cbr-lin-3. Overall, findings program underlying process includes both divergent contributions.
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