KINETICS OF TIAMUTINR INTERACTION WITH CYTOCHROME P-450 FROM RABBIT LIVER
作者： Ingeborg Schuster , Christine Fleschurz , Ilse Helm
DOI: 10.1016/B978-0-08-021523-5.50014-6
关键词: Dissociation rate constant 、 Kinetics 、 Cytochrome 、 Glycerol 、 Stereochemistry 、 Chemistry 、 Liver microsomes 、 Conformational change 、 Reaction rate constant
摘要: The association reaction between Tiamutin R and partially purified Cytochrome P 450 from rabbit liver microsomes gives rise to a TYPE I spectral change can be observed by stopped-flow kinetics. second order rate constant at 25°C in glycerol containing buffer is about 45 μM −1 s , the dissociation 1.1 . This takes also place very high concentrations where equilibrium studies only modified II complex observed. formation of proceeds rather slowly its dependence on -concentration obviously not interpreted terms binding reaction. parallelism emergence unspecific drug attached phospholipids an indication that accumulation lipid bulk might induce conformational site protein.
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