Skin‐draining lymph node priming is sufficient to induce sterile immunity against pre‐erythrocytic malaria

摘要: The Plasmodium-infected hepatocyte has been considered necessary to prime the immune responses leading sterile protection after vaccination with attenuated sporozoites. However, it recently demonstrated that priming also occurs in skin. We wished establish if could be obtained absence of by infected hepatocytes. To this end, we developed a subcutaneous (s.c.) immunization protocol where few, possibly none, immunizing irradiated Plasmodium yoelii sporozoites infect hepatocytes, and used CD81-deficient mice non-permissive productive infections. then compared contrasted patterns those intradermal immunization, liver. Using immunity as primary read-out, exploited an inhibitor T-cell migration, transgenic conditional depletion dendritic cells adoptive transfers draining lymph node-derived T cells, provide evidence can primed skin-draining nodes little, any, contribution from hepatocyte.