Cryopreservation of mouse spermatozoa in the presence of raffinose and glycerol

摘要: When mouse epididymal spermatozoa were rapidly frozen in two steps (37 to -70 degrees C for solid CO2 and -196 liquid nitrogen) as pellets, 18% raffinose provided the greatest protection ICR against cold-shock; sperm motility fertilizing ability 43% 22.4%, respectively. A small proportion of with 10% sucrose was motile but incapable ovulated oocytes. Glycerol dimethylsulphoxide less effective at any concentration examined. However, frozen-thawed significantly improved (35.5%) by addition glycerol (1.75% final concentration) medium containing raffinose. Spermatozoa from one outbred (ddY) 5 inbred (C57BL/6N, C3H/HeN, DBA/2N, BALB/c kk) strains mice successfully presence 1.75% glycerol, although fertilization rates varied among (13% C57BL/6N 64% DBA/2N). fraction eggs resulting developed normally vitro (37% 71% ICR) blastocyst stage vivo (19% ddY oocytes) Day 18 gestation.