Method and kit for amplification, sequencing and typing of classical hla class i genes
作者： Caroline Elizabeth Garey , James Leushner
DOI:
关键词: Biology 、 Exon 、 DNA sequencing 、 Amplicon 、 Single cell sequencing 、 Exon trapping 、 Exome sequencing 、 Multiple displacement amplification 、 Genetics 、 Primer walking
摘要: Amplification primer and sequencing sites have been identified which permit sequence-based typing of each the classical HLA genes in highly robust consistent reactions without allelic dropout. To determine DNA sequence, thus type at least one exon an HLA-A, HLA-B HLA-C gene present a sample, sample is combined with amplification reaction mixture containing primers amplified to form product including 2 3 together single fragment. The then or more oligonucleotide hybridize conserved regions product. between them are effective produce fragments from all known alleles under suitable conditions fragments. evaluated sequence sample.
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