DNA sequence-based HLA class I typing method
作者： Pedro Santamaria , Jose Joaquim Barbosa , Michael Thomas Boyce-Jacino , Stephen Saul Rich , Anthony James Faras
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摘要: The present invention provides a process for determining genotypes in highly polymorphic systems by polymerase chain reaction amplification of cDNA or genomic DNA and direct sequencing products using oligonucleotide primers. More specifically, Class I HLA can be unambiguously determined any subject 16-24 hours HLA-A, HLA-B, HLA-C transcripts enzymatically amplified sequenced limited number selected oligonucleotides.
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