DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR DETERMINATION OF HYDROCHLOROTHIAZIDE, OLMESARTAN MEDOXOMIL AND THEIR RELATED SUBSTANCES IN COMBINED TABLET DOSAGE FORM
作者： Avani B. Chokshi , Pritesh R. Desai , Urvesh Mahendrabhai Patel
DOI:
关键词: Dosage form 、 Chromatographic separation 、 Hydrochlorothiazide 、 Phosphate buffered saline 、 HYDROCHLOROTHIAZIDE/OLMESARTAN 、 Chromatography 、 Chemistry 、 Olmesartan
摘要: Objective: Development of RP-HPLC method for determination Hydrochlorothiazide (HCTZ), Olmesartan medoxomil (OLM) and their related substances in combined tablet dosage form validation the developed method. Methods: Gradient mobile phase system was used estimation drug contents substances. Mobile A contained mixture Acetonitrile 15 mM Phosphate buffer (pH adjusted to 3.4 with orthophosphoric acid) ratio 20:80. B same 80:20. Chromatographic separation carried out at flow rate 0.8 mL/min using C 18 Phenomenax inplace Enable (250 × 4.6 mm) 5 μm column detection made 254 nm. Results: The linearity tested range 62.5-187.5 μg/mL Hydrochlorothiazide, 100-300 medoxomil, 1-1.8 Hydrochlorothiazide. % recovery found be 99.88-100.67 99.14-99.91 (OLM), 99.11-100.71% (HCTZ-IMP) 98.13-100.83% (OLM-IMP). assay marketed formulation 99.78% (HCTZ) 99.26% (OLM). Conclusion: simple, precise accurate
innovareacademics.in参考文章(9)
Abdullah Al Masud, Md Mahfuzur Rahman, Moynul Hasan, Md Kamal Hossain Ripon, Ahsanur Rahman Khan, Md Rabiul Islam, Md Raihan Sarkar, None, Validated Spectrophotometric Method for Estimation of Olmesartan Medoxomil in Pharmaceutical Formulation International Journal of Pharmaceutical and Life Sciences. ,vol. 1, ,(2012) , 10.3329/IJPLS.V1I3.12980
Vipul P. Rane, Kiran R. Patil, Jaiprakash N. Sangshetti, Ravindra D. Yeole, Devanand B. Shinde, Stability-Indicating LC Method for the Determination of Olmesartan in Bulk Drug and in Pharmaceutical Dosage Form Chromatographia. ,vol. 69, pp. 169- 173 ,(2009) , 10.1365/S10337-008-0849-2
O. Sagirli, A. Önal, S. E. Toker, D. Şensoy, Simultaneous HPLC Analysis of Olmesartan and Hydrochlorothiazide in Combined Tablets and in vitro Dissolution Studies Chromatographia. ,vol. 66, pp. 213- 218 ,(2007) , 10.1365/S10337-007-0304-9
A.d Jose, K.G.d Revikumar, V.c Ganesan, M.b Mathew Sr., D.G.T.a Parambi, A VALIDATED HPTLC DETERMINATION OF AN ANGIOTENSIN RECEPTOR BLOCKER OLMESARTAN MEDOXOMIL FROM TABLET DOSAGE FORM. International Journal of Pharmaceutical Sciences Review and Research. ,vol. 4, ,(2010)
Purnima D. Hamrapurkar, Kamalesh K. Gadapayale, Optimization and Validation of Rp-Hplc Stability Indicating Method for Determination of Olmesartan Medoxomil and Its Degraded Product International Journal of Applied Science and Engineering. ,vol. 11, pp. 137- 147 ,(2013) , 10.6703/IJASE.2013.11(2).137
B. N. Vora, R. R. Parmar, D. A. Shah, Absorption Correction Method for Simultaneous Estimation of Metoprolol Succinate and Olmesartan Medoxomil in Combined Tablet Dosage Form ,(2012)
AR Tengli, BM Gurupadayya, Method Development and Validation of Tablet Dosage form Containing Losartan, Atenolol and Hydrochlorthiazide Using Internal Standard by RPHPLC Journal of Chromatography & Separation Techniques. ,vol. 4, pp. 1- 5 ,(2013) , 10.4172/2157-7064.1000180
G. K. Chougule, V. S. Tegeli, G. B. Gajeli, S. T. Kumbhar, Y. S. Thorat, U. S. Shivsharan, A VALIDATED HPTLC METHOD FOR SIMULTANEOUS QUANTIFICATION OF NEBIVOLOL AND HYDROCHLOROTHIAZIDE IN BULK AND TABLET FORMULATION International Journal of Pharmaceutical Sciences and Drug Research. pp. 62- 66 ,(2011)
J. Ashok kumar, K. Senthil kumar, A. Sathya, B. Prathap, Patil sagar. N, V. Gopal, S.B. Lokesh, Simultaneous Estimation of Olmesartan medoxomil and Hydrochlorothiazide by RP-HPLC Method from Combined Dosage Forms International Journal of Research in Pharmaceutical Sciences. ,vol. 1, ,(2010)