The Responses of Tissues from the Brain, Heart, Kidney, and Liver to Resuscitation following Prolonged Cardiac Arrest by Examining Mitochondrial Respiration in Rats.

摘要: Cardiac arrest induces whole-body ischemia, which causes damage to multiple organs. Understanding how each organ responds ischemia/reperfusion is important develop better resuscitation strategies. Because direct measurement of function not practicable in most animal models, we attempt use mitochondrial respiration test efficacy on the brain, heart, kidney, and liver following prolonged cardiac arrest. Male Sprague-Dawley rats are subjected asphyxia-induced for 30 min or 45 min, followed by 60 min cardiopulmonary bypass resuscitation. Mitochondria isolated from tissues examined activity. Following arrest, a time-dependent decrease state-3 observed mitochondria all four tissues. resuscitation, activity brain varies greatly different animals. The after remains same heart significantly increases kidney mitochondria. result shows that inhibition good marker evaluate organ. resulting reenforces need developing strategies resuscitate these critical organs