Effect of local secondary structure of mRNA on expression of recombinant human lymphotoxin in Escherichia coli
作者： Seungkiel Park , Moonhee Sung , Hyangsook Yoo , M. Y. Pack
DOI: 10.1007/BF01021047
关键词: Gene expression 、 Messenger RNA 、 Coding region 、 Escherichia coli 、 Lymphotoxin 、 Gene 、 Protein secondary structure 、 Biology 、 Molecular biology 、 Recombinant DNA
摘要: We substituted a few alternative codons close to the N-terminal coding region of human lymphotoxin (LT) gene for natural ones avoid stable local secondary structures mRNA, predicted in 5′-terminal 130 nucleotides mRNA. With this modification, we have overexpressed recombinant (rLT) inEscherichia coli under control trc promoter. Most produced rLT was soluble and active form.
springer.com
kribb.re.kr参考文章(11)
ED Harlow, David Lane, A Laboratory manual Cold Spring Harbor Laboratory. ,(1989)
Heng-Fong Seow, Cynthia R. Goh, Lata Krishnan, Alan G. Porter, Bacterial Expression, Facile Purification and Properties of Recombinant Human Lymphotoxin (Tumor Necrosis Factor Beta) Nature Biotechnology. ,vol. 7, pp. 363- 368 ,(1989) , 10.1038/NBT0489-363
Anna Mitraki, Jonathan King, Protein folding intermediates and inclusion body formation Nature Biotechnology. ,vol. 7, pp. 690- 697 ,(1989) , 10.1038/NBT0789-690
Patrick W. Gray, Bharat B. Aggarwal, Charles V. Benton, Timothy S. Bringman, William J. Henzel, Julie A. Jarrett, David W. Leung, Barbara Moffat, Peter Ng, Lloyd P. Svedersky, Michael A. Palladino, Glenn E. Nedwin, Cloning and expression of cDNA for human lymphotoxin, a lymphokine with tumour necrosis activity. Nature. ,vol. 312, pp. 721- 724 ,(1984) , 10.1038/312721A0
S. M. Freier, R. Kierzek, J. A. Jaeger, N. Sugimoto, M. H. Caruthers, T. Neilson, D. H. Turner, Improved free-energy parameters for predictions of RNA duplex stability Proceedings of the National Academy of Sciences of the United States of America. ,vol. 83, pp. 9373- 9377 ,(1986) , 10.1073/PNAS.83.24.9373
M. H. de Smit, J. van Duin, Secondary structure of the ribosome binding site determines translational efficiency: a quantitative analysis. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 87, pp. 7668- 7672 ,(1990) , 10.1073/PNAS.87.19.7668
Larry Gold, Posttranscriptional regulatory mechanisms in Escherichia coli. Annual Review of Biochemistry. ,vol. 57, pp. 199- 233 ,(1988) , 10.1146/ANNUREV.BI.57.070188.001215
N. Jacques, M. Dreyfus, Translation initiation in Escherichia coli: old and new questions. Molecular Microbiology. ,vol. 4, pp. 1063- 1067 ,(1990) , 10.1111/J.1365-2958.1990.TB00679.X
Herman A. de Boer, Rob A. Kastelein, CHAPTER 8 – Biased Codon Usage: An Exploration of Its Role in Optimization of Translation Maximizing Gene Expression. pp. 225- 285 ,(1986) , 10.1016/B978-0-409-90027-9.50014-6
Gary D. Stormo, CHAPTER 7 – Translation Initiation Maximizing Gene Expression. pp. 195- 224 ,(1986) , 10.1016/B978-0-409-90027-9.50013-4