Bacterial Expression, Facile Purification and Properties of Recombinant Human Lymphotoxin (Tumor Necrosis Factor Beta)

摘要: We have expressed a synthetic recombinant human TNF–β(rhTNF–β) gene in Escherichia coli to give rhTNF–β soluble, highly active and intact form. The specific activity of purified single, convenient step from bacterial lysates was 3–5 × 107 units/mg on mouse L–929 cells. cytostatic (growth inhibitory) cytotoxic (cell killing) activities were compared variety untransformed transformed cell lines. In the absence protein synthesis inhibitor cycloheximide, two lines growth inhibited by but none lysed. presence five out eleven became sensitive lysis rhTNF–β, indicating that some not all cells require uninterrupted order protect themselves TNF–β cytotoxicity. Cell varied greatly their sensitivity different pattern response seen assays.