Diverse and Abundant Secondary Metabolism Biosynthetic Gene Clusters in the Genomes of Marine Sponge Derived Streptomyces spp. Isolates

摘要: The genus Streptomyces produces secondary metabolic compounds that are rich in biological activity. Many of these genetically encoded by large metabolism biosynthetic gene clusters (smBGCs) such as polyketide synthases (PKS) and non-ribosomal peptide synthetases (NRPS) which modular can be highly repetitive. Due to the repeats, difficult resolve using short read next generation datasets often quite poorly predicted standard approaches. We have sequenced genomes 13 spp. strains isolated from shallow water deep-sea sponges display antimicrobial activities against a number clinically relevant bacterial yeast species. Draft been assembled smBGCs identified antiSMASH (antibiotics Secondary Metabolite Analysis Shell) web platform. compared amongst search for novel sequences conferring potential produce bioactive metabolites. this study recruit four distinct clades within Streptomyces. marine host abundant encode polyketides, NRPS, siderophores, bacteriocins lantipeptides. appear enriched with encoding NRPS. Marine adaptations evident sponge-derived genes involved biosynthesis transport compatible solutes heat-shock proteins. environments promising source metabolites abundance diversity show high degrees novelty. Sponge derived isolates genomic living when terrestrial strains.