miRNAs: A New Method for Erythroid Differentiation of Hematopoietic Stem Cells Without the Presence of Growth Factors

摘要: Micro RNAs (miRNAs) are a novel class of non-coding regulatory RNA molecules that contribute to post-transcriptional gene regulation. Recent studies have demonstrated specific miRNAs such as miR-150, miR-154, and miR-451 key roles in erythropoiesis. To date, stimulatory cytokines considered unique effectors for vitro differentiation HSCs erythropoietic lineage. However, the use these factors is not cost-effective clinical applications therapeutic strategies. Here, we present strategy which expression modulation promotes erythroid absence any extrinsic factors. Thus, CD133(+) hematopoietic stem cells purified from human umbilical cord blood were treated with pre-miR-451 containing lentiviruses, anti-miR-150 anti-miR-154 growth cytokines. Obtained results indicated upregulation miR-150 downregulation positive effect on GATA-1, FOG-1, EKLF, CD71 CD235a genes induce hemoglobinization efficiently. miR-154 had no erythropoiesis indexes compared observed control group. In conclusion, data presented here first time demonstrate could be an efficient alternative into Modulation via miRNA holds promising potential vascular tissue engineering regenerative medicine applications.