"One-" and '"Two-dimensional" Partition Chromatographic Separations of Organic Acids on an Inert Sheet Support

摘要: Procedures have been described for "one-" and "two-dimensional" partition-chromatographic separations, on an inert sheet (paper) support, of mixtures relatively non-volatile organic acids. Ionization, adsorption the acids by paper, suppressed swamping both stationary mobile solvent phases with a volatile acid (acetic or formic). Formic is superior in that, at high concentration, it permits welldefined bands test to travel down sheets confers upon highly characteristic RF values. values some (malic, citric, tartaric, succinic, fumaric, malonic, pyruvic, lactic, ketosuccinic (oxalacetic), α-ketoglutaric, monomethyl glutaric, adipic) 20°C., when Whatman No. 1 filter paper various pairs are used. provided. Some present carrot tissue separated "one-dimensional" work identified their