Chapter 9 Culture media for the isolation of campylobacters

摘要: Abstract The history of the development selective media for isolation campylobacters, including rationale choice agents is described. Developments have included modifications to allow incubation at 37°C instead 42 or 43°C and changes in types concentrations antibiotics order not inhibit organisms such as Campylobacter upsaliensis, C. jejuni subsp. doylei some strains coli lari . When examining foods, plating originally developed from faeces are normally used, sometimes after liquid enrichment. Most include ingredients intended protect campylobacters toxic effect oxygen derivatives. commonly used lysed defibrinated blood; charcoal; a combination ferrous sulphate, sodium metabisulphite pyruvate (FBP); haemin haematin. To date no medium includes an indicator system — instance pH show whether colonies produce acid alkali particular substrates. manner which enrichment has been modified food samples avoid inhibitory effects on sublethally damaged cells by components formula. This done preliminary period reduced temperature delayed addition antibiotics. Expensive time-consuming methods proposed achieve microaerobic atmosphere while using media. there generally accepted ‘standard’ method isolating food.