The relationship between graft incompatibility and phenols in Uapaca kirkiana Müell Arg.

作者: Simon A. Mng’omba , Elsa S. du Toit , Festus K. Akinnifesi

DOI: 10.1016/J.SCIENTA.2008.03.031

关键词: BiochemistryPhenolExudateCallus formationChromatographyRootstockUapaca kirkianaCallusFerulic acidChemistryPhenols

摘要: The objective of the study was to determine relationship between phenols and graft incompatibility in Uapaca kirkiana. Phenol quantification identification were carried out using Folin-Ciocalteau reagent procedure, fluorescence microscopy reverse phase high performance liquid chromatograph (RP-HPLC) above, below at union. Results showed no vascular cambium continuity above scion/stock unions. Significant differences total soluble cell wall bound obtained. Fluorescence microscope indicated presence flavonoids other polymers RP-HPLC identified ferulic acid as a major phenol component found U. kirkiana plant cells responsible for wood discolouration. High concentrations obtained less compatible combinations than combinations. peaks r-coumaric It is concluded that phenols, especially acids caused poor callus formation union, hence implicated incompatibility.

参考文章(24)
N. Kangatharalingam, Margaret L. Pierce, Melanie B. Bayles, Margaret Essenberg, Epidermal anthocyanin production as an indicator of bacterial blight resistance in cotton Physiological and Molecular Plant Pathology. ,vol. 61, pp. 189- 195 ,(2002) , 10.1006/PMPP.2002.0434
J. B. Harborne, P. M. Dey, Methods in plant biochemistry Academic Press. ,(1989)
J. Méndez, M.D.V. Gesto, A. Vázquez, E. Vieitez, E. Seoane, Growth substances isolated from woody cuttings of Alnus glutinosa medic. and Fraxinus excelsior L. Phytochemistry. ,vol. 7, pp. 575- 579 ,(1968) , 10.1016/S0031-9422(00)88229-1
Ana Pina, Pilar Errea, A review of new advances in mechanism of graft compatibility-incompatibility Scientia Horticulturae. ,vol. 106, pp. 1- 11 ,(2005) , 10.1016/J.SCIENTA.2005.04.003
V. Usenik, B. Krška, M. Vičan, F. Štampar, Early detection of graft incompatibility in apricot (Prunus armeniaca L.) using phenol analyses Scientia Horticulturae. ,vol. 109, pp. 332- 338 ,(2006) , 10.1016/J.SCIENTA.2006.06.011
P. Errea, A. Felipe, M. Herrero, Graft establishment between compatible and incompatible Prunus spp Journal of Experimental Botany. ,vol. 45, pp. 393- 401 ,(1994) , 10.1093/JXB/45.3.393