Cytotoxic Activities of Physalis minima L. Chloroform Extract on Human Lung Adenocarcinoma NCI-H23 Cell Lines by Induction of Apoptosis
作者： Ooi Kheng Leong , Tengku Sifzizul Tengku Muhammad , Shaida Fariza Sulaiman
DOI: 10.1093/ECAM/NEP057
关键词: Molecular biology 、 Apoptosis 、 Cell culture 、 Annexin 、 Propidium iodide 、 Cytotoxic T cell 、 Pathology 、 Staining 、 DNA fragmentation 、 Programmed cell death 、 Biology
摘要: Physalis minima L. is reputed for having anticancer property. In this study, the chloroform extract of plant exhibited remarkable cytotoxic activities on NCI-H23 (human lung adenocarcinoma) cell line at dose- and time-dependent manners (after 24, 48 72 h incubation). Analysis cell-death mechanism demonstrated that exerted apoptotic programed death in cells with typical DNA fragmentation, which a biochemical hallmark apoptosis. Morphological observation using transmission electron microscope (TEM) also displayed characteristics treated cells, including clumping margination chromatins, followed by convolution nuclear budding to produce membrane-bound bodies. Different stages as well phosphatidylserine externalization were confirmed annexin V propidium iodide staining. Furthermore, acute exposure produced significant regulation c-myc, caspase-3 p53 mRNA expression line. Due its effect it strongly suggested could be further developed an drug.
hindawi.com参考文章(36)
R. Overbeeke, H. Steffens-Nakken, I. Vermes, C. Reutelingsperger, C. Haanen, Early features of apoptosis detected by four different flow cytometry assays. Apoptosis. ,vol. 3, pp. 115- 121 ,(1998) , 10.1023/A:1009649025439
G Koopman, CP Reutelingsperger, GA Kuijten, RM Keehnen, ST Pals, MH van Oers, Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis. Blood. ,vol. 84, pp. 1415- 1420 ,(1994) , 10.1182/BLOOD.V84.5.1415.1415
Hideki Sakahira, Masato Enari, Shigekazu Nagata, Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis Nature. ,vol. 391, pp. 96- 99 ,(1998) , 10.1038/34214
John F. R. Kerr, Clay M. Winterford, Brian V. Harmon, Apoptosis. Its significance in cancer and cancer Therapy Cancer. ,vol. 73, pp. 2013- 2026 ,(1994) , 10.1002/1097-0142(19940415)73:8<2013::AID-CNCR2820730802>3.0.CO;2-J
Wen-Tsong Hsieh, Kuan-Yuh Huang, Hui-Yi Lin, Jing-Gung Chung, Physalis angulata induced G2/M phase arrest in human breast cancer cells. Food and Chemical Toxicology. ,vol. 44, pp. 974- 983 ,(2006) , 10.1016/J.FCT.2005.11.013
Li Lin, Peter L. Hwang, Antiproliferative effects of oxygenated sterols: positive correlation with binding affinities for the antiestrogen-binding sites. Biochimica et Biophysica Acta. ,vol. 1082, pp. 177- 184 ,(1991) , 10.1016/0005-2760(91)90192-K
Lorne J Hofseth, S.Perwez Hussain, Curtis C Harris, p53: 25 years after its discovery. Trends in Pharmacological Sciences. ,vol. 25, pp. 177- 181 ,(2004) , 10.1016/J.TIPS.2004.02.009
Takahiro Hosoya, Midori A. Arai, Takashi Koyano, Thaworn Kowithayakorn, Masami Ishibashi, Naturally occurring small-molecule inhibitors of hedgehog/GLI-mediated transcription. ChemBioChem. ,vol. 9, pp. 1082- 1092 ,(2008) , 10.1002/CBIC.200700511
Hui-Fen Chiu, Tsai-Tung Chih, Yun-Mei Hsian, Chih-Hua Tseng, Ming-Jung Wu, Yang-Chang Wu, Bullatacin, a potent antitumor Annonaceous acetogenin, induces apoptosis through a reduction of intracellular cAMP and cGMP levels in human hepatoma 2.2.15 cells. Biochemical Pharmacology. ,vol. 65, pp. 319- 327 ,(2003) , 10.1016/S0006-2952(02)01554-X
John D Benson, Ying-Nan P Chen, Susan A Cornell-Kennon, Marion Dorsch, Sunkyu Kim, Magdalena Leszczyniecka, William R Sellers, Christoph Lengauer, None, Validating cancer drug targets Nature. ,vol. 441, pp. 451- 456 ,(2006) , 10.1038/NATURE04873