Physalis angulata induced G2/M phase arrest in human breast cancer cells.
作者： Wen-Tsong Hsieh , Kuan-Yuh Huang , Hui-Yi Lin , Jing-Gung Chung
DOI: 10.1016/J.FCT.2005.11.013
关键词:
摘要: Physalis angulata (PA) is employed in herbal medicine around the world. It used to treat diabetes, hepatitis, asthma and malaria Taiwan. We have evaluated PA as a cancer chemopreventive agent vitro by studying role of regulation proliferation, cell cycle apoptosis human breast lines. inhibited proliferation induced G2/M arrest MAD-MB 231 MCF-7 In this study, under treatment with various concentrations MDA-MB line, we checked mRNA levels for cyclin A B1 protein B1, Cdc2 (cyclin-dependent kinases), p21(waf1/cip1) P27(Kip1) kinase inhibitors), Cdc25C, Chk2 Wee1 relative factors) phase. From those results, determined that arrests cells at phase (i) inhibiting synthesis or stability their downstream (ii) increasing P27(kip1) levels, (iii) Chk2, thus causing an increase Cdc25C phosphorylation/inactivation inducing decrease level. According results obtained, appears possess anticarcinogenic properties; these suggest effect on phosphorylated/inactivated are mediated activation, least part, via cyclin-dependent inhibitors pathway carcinoma cells.
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sci-hub.se 参考文章(44)
Mark J. Jaroszeski, Richard Heller, Flow cytometry protocols Humana Press. ,(1997) , 10.1385/0896033546
H C Chiang, W S Kan, C F Chen, S M Jaw, Antitumor agent, physalin F from Physalis angulata L Anticancer Research. ,vol. 12, pp. 837- 843 ,(1992)
Michael G. Ormerod, Flow cytometry : a practical approach IRL Press at Oxford University Press. ,(1994)
Waldemar Priebe, Yi-He Ling, Roman Perez-Soler, Apoptosis Induced by Anthracycline Antibiotics in P388 Parent and Multidrug-resistant Cells Cancer Research. ,vol. 53, pp. 1845- 1852 ,(1993)
M Sikorska, J F Whitfield, P R Walker, T Youdale, C Smith, J Leblanc, Topoisomerase II-reactive chemotherapeutic drugs induce apoptosis in thymocytes. Cancer Research. ,vol. 51, pp. 1078- 1085 ,(1991)
Jennifer A. Pietenpol, Caroline D. Scatena, Liying Yang, Holly A. Goodman, Steven D. Leach, Christopher J. Keefer, Si Young Song, Negative Regulation of Wee1 Expression and Cdc2 Phosphorylation during p53-mediated Growth Arrest and Apoptosis Cancer Research. ,vol. 58, pp. 3231- 3236 ,(1998)
Helen Piwnica-Worms, Christine E. Canman, Joon Young Ahn, Julie K. Schwarz, Threonine 68 phosphorylation by ataxia telangiectasia mutated is required for efficient activation of Chk2 in response to ionizing radiation Cancer Research. ,vol. 60, pp. 5934- 5936 ,(2000)
Tony Hunter, When is a lipid kinase not a lipid kinase? When it is a protein kinase Cell. ,vol. 83, pp. 1- 4 ,(1995) , 10.1016/0092-8674(95)90225-2
L Shi, W. Nishioka, J Th'ng, E. Bradbury, D. Litchfield, A. Greenberg, Premature p34cdc2 activation required for apoptosis Science. ,vol. 263, pp. 1143- 1145 ,(1994) , 10.1126/SCIENCE.8108732
Katharine O Hartley, David Gell, Graeme C.M Smith, Hong Zhang, Nullin Divecha, Margery A Connelly, Arie Admon, Susan P Lees-Miller, Carl W Anderson, Stephen P Jackson, DNA-dependent protein kinase catalytic subunit: A relative of phosphatidylinositol 3-kinase and the ataxia telangiectasia gene product Cell. ,vol. 82, pp. 849- 856 ,(1995) , 10.1016/0092-8674(95)90482-4