Biology, growth parameters and enzymatic activity of Pochonia chlamydosporia isolated from potato cyst and root-knot nematodes

摘要: Pochonia chlamydosporia, a widespread fungal parasite of potato cyst nematodes (PCN), Globodera spp., and root-knot (RKN), Meloidogyne has been studied as biological control agent. Three Portuguese isolates (Pc1, Pc2, Pc3) obtained from PCN eggs two non-native (Vc10, Pc280) were characterised using ERIC-PCR screened by in vitro assays for their ability to produce chlamydospores, parasitise rostochiensis chitwoodi colonise the rhizosphere barley. The effects temperature on growth, sporulation, parasitism enzymatic activity also evaluated. Isolates Pc1 Pc3, despite different geographical origins, had identical molecular profiles. Pc2 produced higher numbers chlamydospores solid medium (1.15 × 107 g−1), whereas Pc3 least (3 105 g−1). These extensively colonised barley (>90% root fragments) proportion parasitised eggs, detected agar plates, was low (<60% RKN <55% PCN), being best against both nematode species. influence similar all isolates: no growth observed at 10, 33 35°C. Spores/hyphal fragments remained viable nearly 1 month 10 33°C resumed after incubation 25°C, although conidia production, viability egg affected. Exposure 35°C lethal Pc1, Pc280. When grown liquid media esterases, but protease only Vc10. highest isolate nutrient media. Enzymatic decreased presence Molecular, biochemical analyses, including biotic abiotic factors, are important screening potential agents, particularly case P. due high variability among isolates.