Gliding Motility and Expression of Motility-Related Genes in Spreading and Non-spreading Colonies of Flavobacterium columnare

摘要: Gliding motility facilitates the movement of bacteria along surfaces in many Bacteroidetes species and results spreading colonies. The adhesins required for gliding are secreted through a motility-associated protein secretion system, known as type IX system (T9SS). fish pathogen Flavobacterium columnare produces (rhizoid [Rz], soft [S]) non-spreading (rough [R]) colony types, which only Rz is virulent. In this study, we explored behavior these types by microscopic imaging measured expression genes associated with T9SS (gldG, gldH, gldL, sprA, sprB, sprE, sprF, sprT, porV) under high low resource levels using RT-qPCR (reverse transcription quantitative PCR). responded to level increased size. type, well cells growing nutrient expressed moderate cell movements. Yet, provoked more active individual cooperative gliding. gene survey demonstrated an sprA (a core component T9SS) sprF (needed adhesin secretion) conditions. Surprisingly, was not consistently behavior. Furthermore, no genetic differences were found between studied motility. Our study demonstrates that environmental important regulator both some genes. These may help understand connections concentration, motility, virulence F. columnare.