Evaluation of the genotoxic potential of the Casearia sylvestris extract on HTC and V79 cells by the comet assay
作者： E.L. Maistro , J.C.T. Carvalho , M.S. Mantovani
DOI: 10.1016/J.TIV.2003.10.002
关键词: V79 cells 、 Tissue culture 、 Chinese hamster 、 Casearia sylvestris 、 Traditional medicine 、 Cell culture 、 Comet assay 、 Methyl methanesulfonate 、 Biology 、 DNA damage
摘要: Casearia sylvestris is common in tropical America growing wild Brazil the states of Amazonas and Sao Paulo. Its leaves are used Brazilian folk medicine for several diseases. The present investigation was carried out to examine genotoxic effects a C. crude ethanolic extract on Hepatoma Tissue Culture (HTC cells) Rattus norvegicus Chinese hamster V79 cells culture, using comet assay. For evaluation were treated with three different concentrations (0.5, 1 2 mg/ml) prepared from 25 mg/ml aqueous solution. positive control cyclophosphamide HTC methyl methanesulfonate cells. duration treatment h. results showed that presented no not modified effect inducing DNA damage by alkylating agents V 79 respectivelly.
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