Biosynthesis of the labdane diterpene marrubiin in Marrubium vulgare via a non-mevalonate pathway.

摘要: The biosynthesis of the furanic labdane diterpene marrubiin has been studied in plantlets and shoot cultures Marrubium vulgare (Lamiaceae). use [2-14C]acetate, [2-14C]pyruvate, [2-14C]mevalonic acid [U-14C]glucose incorporation experiments showed that labelling sterols etiolated M. was accordance with their via acetate-mevalonate pathway. In contrast, rates these precursors into could not be explained by this compound Cultivation on medium containing [1-13C]glucose subsequent 13C-NMR spectroscopy led to conclusion follows a non-mevalonate All isoprenic units 13C-labelled were enriched those carbons correspond positions 1 5 putative precursor isopentenyl diphosphate. This pattern from is consistent an alternative pathway trioses, which already shown occur Eubacteria Gymnospermae. skeleton many other skeletal types diterpenes. Therefore it becomes obvious connection few known examples isoprenoids formation some plants might quite common.