Translocation of isopentenyl pyrophosphate for Taxol biosynthesis in suspension cultures of Taxus chinensis var. mairei
作者： Yan-Dong Wang
关键词: Biochemistry 、 Biology 、 Isopentenyl pyrophosphate 、 Metabolite 、 Cytoplasm 、 Taxus 、 Pyrophosphate 、 Plastid 、 Plant physiology 、 Chromosomal translocation
摘要: Two inhibitors of metabolite translocators, sodium pyrophosphate (NaPP) and D,L-glyceraldehyde (DLG), respectively, were added into suspension cultures Taxus chinensis var. mairei at the early late growth phases to investigate translocation isopentenyl (IPP) from cytoplasm plastids for Taxol biosynthesis. NaPP DLG hardly affected cell biomass viability regardless their introduction. The content varied less when or was day 7 but decreased obviously introduced 14. It is thus inferred that inhibited biosynthesis by blocking IPP phase, suggesting be involved only phase cells.
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