Source of isopentenyl diphosphate for taxol and baccatin III biosynthesis in cell cultures of Taxus baccata

摘要: Abstract To achieve a better understanding of the metabolism and accumulation taxol baccatin III in cell cultures Taxus , three lines (I, II III) T. baccata were treated (on day 7) with several concentrations fosmidomycin (100, 200 300 μM), an inhibitor non-mevalonate branch terpenoid pathway, or mevinolin (1, 3 5 μM), mevalonate branch, both cases presence absence 100 μM methyl jasmonate (MeJ). They compared only elicitor MeJ as well untreated control respect to growth, viability production III. The results show that line type was important variable, mainly for taxane accumulation. blocking effect on significantly greater than all lines, clearly suggesting isopentenyl diphosphate (IPP) used ring formation formed via pathway. However, significant reduction content average 3.8-fold) 4.3-fold) I when together 5 1 μM, respectively, also suggests pathways are involved biosynthesis two taxanes result cytosolic IPP and/or other prenyl transport plastids. observation inhibitory mevilonin baccatinn yield does not interfere elicitation is discussed.