Metabolic fluxes and l-lysine synthesis by Corynebacterium glutamicum in relation to cellular total reducing activity
作者： Maija Ruklisha , Longina Paegle
DOI: 10.1016/S0032-9592(01)00165-0
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摘要: Abstract The total reducing activity (TRA) of cells was used to estimate the physiological Corynebacterium glutamicum under conditions l -lysine synthesis. This estimated as rate reduction 2,3,5- triphenyltetrazolium chloride by intact cells. TRA linearly correlated with intracellular concentrations RNA and bacterial growth rate. It concluded that this reflected energy generation in A decrease growing related an increase lysine synthesis activity. Alteration metabolic pathway functioning precursors favoured when cellular decreasing.
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