Expression and purification of an HTLV-III gag/env gene protein
作者： Robert Mitchell Crowl , Daru Young
DOI:
关键词: Novel protein 、 Gene 、 Cloning 、 Antibody 、 Biology 、 Immunization 、 Htlv iii 、 Virology 、 Antigen 、 Plasmid 、 Molecular biology
摘要: Methods and compositions are provided for the cloning expression of plasmids bearing genes encoding a novel protein derived from HTLV-III. This protein, which is called gag/env contains antigenic determinants both core envelope proteins HTLV-III, can be purified to homogeneity used as basis diagnostic tests detect presence antibodies against viruses associated with AIDS or themselves in human sera other biological fluids. The may also formulated use vaccine protection through prophylactic immunization.
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