A genetically encoded probe for imaging HA-tagged protein translation, localization, and dynamics in living cells and animals
作者： Ning Zhao , Kouta Kamijo , Philip D. Fox , Haruka Oda , Tatsuya Morisaki
DOI: 10.1101/474668
关键词: Translation (biology) 、 Single-chain variable fragment 、 Cytoplasm 、 Membrane protein 、 Biology 、 Cell biology 、 Epitope 、 In vivo 、 Messenger RNA 、 Intracellular
摘要: ABSTRACT To expand the toolbox of imaging in living cells, we have engineered a new single chain variable fragment (scFv) that binds classic linear HA epitope with high affinity and specificity vivo. The resulting probe, which call frankenbody, is capable lighting up multiple colors HA-tagged nuclear, cytoplasmic, membrane proteins diverse cell types. frankenbody also enables state-of-the-art single-molecule experiments, demonstrate by tracking mRNA translation dynamics U2OS cells neurons. In combination SunTag, track two species simultaneously to comparative studies can now be done genetically encoded tools alone. Finally, use precisely quantify expression tagged developing zebrafish embryos. versatility makes it powerful tool for protein One-sentence summary A encodable intracellular single-chain selectively (YPYDVPDYA) organisms used translation, localization, dynamics.
sci-hub.st 参考文章(60)
Jörg Burmester, Andreas Plückthun, Construction of scFv Fragments from Hybridoma or Spleen Cells by PCR Assembly Springer, Berlin, Heidelberg. pp. 21- 44 ,(2001) , 10.1007/978-3-662-04605-0_2
Hiroshi Kimura, Yoko Hayashi-Takanaka, Timothy J. Stasevich, Yuko Sato, Visualizing posttranslational and epigenetic modifications of endogenous proteins in vivo. Histochemistry and Cell Biology. ,vol. 144, pp. 101- 109 ,(2015) , 10.1007/S00418-015-1344-0
R R Porter, The hydrolysis of rabbit γ-globulin and antibodies with crystalline papain Biochemical Journal. ,vol. 73, pp. 119- 127 ,(1959) , 10.1042/BJ0730119
Michael S. Packer, David R. Liu, Methods for the directed evolution of proteins Nature Reviews Genetics. ,vol. 16, pp. 379- 394 ,(2015) , 10.1038/NRG3927
Nicola Green, Hannah Alexander, Arthur Olson, Stephen Alexander, Thomas M. Shinnick, J.Gregor Sutcliffe, Richard A. Lerner, Immunogenic structure of the influenza virus hemagglutinin Cell. ,vol. 28, pp. 477- 487 ,(1982) , 10.1016/0092-8674(82)90202-1
Sarada Viswanathan, Megan E Williams, Erik B Bloss, Timothy J Stasevich, Colenso M Speer, Aljoscha Nern, Barret D Pfeiffer, Bryan M Hooks, Wei-Ping Li, Brian P English, Teresa Tian, Gilbert L Henry, John J Macklin, Ronak Patel, Charles R Gerfen, Xiaowei Zhuang, Yalin Wang, Gerald M Rubin, Loren L Looger, High-performance probes for light and electron microscopy Nature Methods. ,vol. 12, pp. 568- 576 ,(2015) , 10.1038/NMETH.3365
Yuko Sato, Masanori Mukai, Jun Ueda, Michiko Muraki, Timothy J. Stasevich, Naoki Horikoshi, Tomoya Kujirai, Hiroaki Kita, Taisuke Kimura, Seiji Hira, Yasushi Okada, Yoko Hayashi-Takanaka, Chikashi Obuse, Hitoshi Kurumizaka, Atsuo Kawahara, Kazuo Yamagata, Naohito Nozaki, Hiroshi Kimura, Genetically encoded system to track histone modification in vivo. Scientific Reports. ,vol. 3, pp. 2436- 2436 ,(2013) , 10.1038/SREP02436
J. M. Halstead, T. Lionnet, J. H. Wilbertz, F. Wippich, A. Ephrussi, R. H. Singer, J. A. Chao, An RNA biosensor for imaging the first round of translation from single cells to living animals Science. ,vol. 347, pp. 1367- 1671 ,(2015) , 10.1126/SCIENCE.AAA3380
Jonas Ries, Charlotte Kaplan, Evgenia Platonova, Hadi Eghlidi, Helge Ewers, A simple, versatile method for GFP-based super-resolution microscopy via nanobodies Nature Methods. ,vol. 9, pp. 582- 584 ,(2012) , 10.1038/NMETH.1991
Philipp Holliger, Peter J Hudson, Engineered antibody fragments and the rise of single domains Nature Biotechnology. ,vol. 23, pp. 1126- 1136 ,(2005) , 10.1038/NBT1142