Weightlessness affects cytoskeleton of rat utricular hair cells during maturation in vitro.
作者： Sophie Gaboyard , Marie-Pierre Blanchard , C??cile Travo , Michel Viso , Alain Sans
DOI: 10.1097/00001756-200211150-00030
关键词: In vitro maturation 、 Immunocytochemistry 、 Vestibular Hair Cell 、 Cell biology 、 Calretinin 、 Cytoskeleton 、 Inner ear 、 Biology 、 Internal medicine 、 Weightlessness 、 Hair cell 、 Endocrinology
摘要: The aim of this study was to investigate whether an altered gravitational environment affected the phenotype vestibular hair cells during in vitro maturation. We developed, using automated incubator, a 3D culture utricles from newborn rats. These cultures were subjected weightlessness for 1 or 3 days, and then compared with control developed natural induced 1G gravity. Immunocytochemistry α-tubulin calretinin revealed disorganisation microtubules loss cell shape conclude that lack strain cytoskeletal dynamics, resulting specific morphological cells.
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