Demonstration of feasibility of automated osteoblastic line culture in space flight

摘要: There is a large body of evidence that microgravity- or immobilization-induced bone loss mainly related to osteoblastic cell impairment. Osteoblasts are sensitive increased mechanical stress and could therefore be responsible for unloading-induced changes. However, the nature osteoblast involvement remains unclear. The effects space environment on cells have been studied extensively, but little information about anchorage-dependent cultures 25 different types flown in has published. We long-term weightlessness shape cultured osteoblasts during Russian Bion 10 space-flight. This experiment required development special automatic culture devices (the plunger-box system) finalized with constructors. Multiple feasibility experiments were performed allow 6 days microgravity. study revealed biocompatibility; optimization ROS 17/2.8 (mammalian adherent cells) under closed conditions (without gas exchange); transport viable 5 days. During microgravity, growth curves ground controls roughly similar. Alkaline phosphatase activity was enhanced twofold morphology began change significantly after 4 microgravity; they became rounder covered microvilli. At end flight, exhibited mixed morphological types, piling cells, stellar shape, spread out resembling 1g flight (centrifuge). demonstrated day conditions. They adapted their structure function this environment, characterized by stimuli.