Early-life sleep deprivation persistently depresses melatonin production and bio-energetics of the pineal gland: potential implications for the development of metabolic deficiency

摘要: Early-life sleep deprivation (ESD) is a serious condition with severe metabolic sequelae. The pineal hor- mone melatonin plays an important role in homeostatic regulation of function. Considering norepi- nephrine-mediated Ca 2? influx and subsequent protein kinase A (PKA) activation responsible for downstream cAMP-response element-binding (CREB) phos- phorylation biosynthesis, the present study determined whether expression, together molecular machinery participated production would significantly alter after ESD. Weaning rats subjected to chronic ESD maintained naturally (light:dark cycle = 12:12) adulthood were processed time-of- flight secondary ion mass spectrometry, immunoblotting, immunohistochemistry spectrometric assay detect signaling, adrenoreceptors, PKA, phosphorylated CREB (pCREB) as well serum level melatonin, respectively. Pineal bio-energetics met- abolic function by measuring cyto- chrome oxidase activity glucose, triglyceride, insulin, high- low-density lipoproteins, Results indicated that normal rats, strong signaling along intense pCREB activities all detected pinealocytes. Enhanced imaging pathway corre- sponded intact bio-energetics, activity. However, following ESD, not only but also decreased. Blood analysis showed reduced impaired As depressed -mediated biosynthesis are positively correlated development dysfunction, supplementary use childhood may thus serve practical way prevent or counteract ESD-induced deficiency.