Fibrinogen-bearing protein genes in the snail Biomphalaria glabrata: Characterization of two novel genes and expression studies during ontogenesis and trematode infection

摘要: All fibrinogen (FBG)-bearing proteins documented to date in the freshwater snail Biomphalaria glabrata, intermediate host of human blood fluke Schistosoma mansoni, possess same molecular structure; one or two immunoglobin superfamily (IgSF) domains at N-terminus and a FBG domain C-terminus (named as FBG-related protein (FREP)). Here we report novel genes that encode FBG-bearing from B. glabrata. Different all known FREPs, first gene encodes (657 amino acids (aa)) composed long N-terminal region with no sequence homology any protein, middle epidermal growth factor (EGF) repeat C-terminal domain, designated molecule (FReM). Differential expression 2 days post-exposure (dpe) trematode S. mansoni Echinostoma paraensei was found susceptible M line resistant BS-90 strains. The second is new member FREP family, FREP14, which 399 aa putative secreted protein. FREP14 different FREPs it encoded by single locus not upregulated early late stage exposure, but E. infection. Furthermore, during snail's ontogenesis infection (52 dpe) has been investigated newly identified (FReM FREP14) described this paper five representative members (FREPs 2, 3, 4, 12, 13). A variety patterns were observed, suggestive functional diversity among proteins. Our findings further broaden our understanding function