Biomarker Mapping on Skin Tape Strips Using MALDI Mass Spectrometry Imaging.

摘要: Keratinocyte organization and biochemistry are important in forming the skin’s protective barrier. Intrinsic extrinsic factors can affect skin barrier function at cellular molecular levels. Matrix-assisted laser desorption/ionization (MALDI) mass spectrometric imaging, a technique which combines both aspects histological details, has proven to be valuable method various disciplines including pharmacology, dermatology cosmetology. It typically requires ex vivo samples, prepared following frozen tissue sectioning. This paper demonstrates feasibility of performing MALDI analysis on tape strips collected non-invasively skin. The aim is obtain imaging corneocytes tapes towards novel biological insights. Tapes were from two sites (volar forearm cheek) human volunteers. Ten molecules relating detected with single mode acquisition high spatial resolution 7 T MALDI-Fourier transform ion cyclotron resonance (FTICR) instrument. sensitivity was adequate create maps could overlaid transmission microscopy images same area tape. Analysis distributions consistent known properties sites, confirming validity observations. Hierarchical clustering used differentiate corneocyte populations based their profiles. Furthermore, morphological provided new way considering statistical tape, rather than measuring averaged value, providing additional useful information structure-function relationship.