Quantitative lipidomic analysis of Ascaris suum.

摘要: Ascaris is a soil-transmitted nematode that causes ascariasis, neglected tropical disease affecting predominantly children and adolescents in the tropics subtropics. Approximately 0.8 billion people are affected worldwide, equating to 0.86 million disability-adjusted life-years (DALYs). Exploring molecular biology of important gain better understanding host-parasite interactions processes, supports development novel interventions. Although advances have been made genomics, transcriptomics proteomics Ascaris, its lipidome has received very limited attention. Lipidomics an sub-discipline systems biology, focused on exploring lipids profiles tissues cells, elucidating their biological metabolic roles. Here, we characterised lipidomes key developmental stages organ porcine origin via high throughput LC-MS/MS. In total, > 500 lipid species belonging 18 classes within three categories were identified quantified–in precise molar amounts relation dry weight worm material–in different stages/sexes systems. The results showed substantial differences composition abundance with roles cellular processes functions (e.g. energy storage regulation membrane structure) among distinct systems, likely reflecting differing demands for lipids, depending stage growth as well need adapt constantly changing environments outside host animal. This work provides first step toward possibilities designing new interventions against ascariasis.