Culture of Tumor Cells
作者： R. Ian Freshney
DOI: 10.1002/0471747599.CAC024
关键词: Cervix 、 Cell culture 、 Carcinoma 、 Cryopreservation 、 Pancreas 、 Glioma 、 Prostate 、 Biology 、 Ovary 、 Pathology
摘要: Following a discussion of the general problems tumor cell culture, broad approach to sampling, disaggregation, primary culture and characterization is described, with additional information on generation continuous lines selective methods. A number specific types are covered protocols for colorectal tumors, use confluent monolayers, freezing biopsies. Several other reviewed appropriate references literature. Keywords: cells; carcinoma; glioma; feeder layers; lines; culture; cryopreservation; breast; lung; stomach; pancreas; ovary; prostate; bladder; skin; cervix
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