Progesterone inhibits contraction and increases TREK-1 potassium channel expression in late pregnant rat uterus

摘要: // Zongzhi Yin 1, 3, 4 , Yun Li 1 Wenzhu He Dan 5 Hongyan Yuanyuan Yang Bing Shen 6 Xi Wang 7 Yunxia Cao 2, and Raouf A. Khalil Department of Obstetrics Gynecology, The First Affiliated Hospital Anhui Medical University, Hefei, China 2 Reproductive Medicine Center, 3 Province Key Laboratory Health Genetics, Provincial Engineering Technology Research Center for Biopreservation Artificial Organs, Scientific Research, Second Physiology, Vascular Surgery Laboratories, Division Endovascular Surgery, Brigham Women’s Hospital, Harvard School, Boston, MA, USA Correspondence to: Khalil, email: raouf_khalil@hms.harvard.edu Cao, caoyunxia6@126.com Keywords: pregnancy; uterine contraction; progesterone; TREK-1 channel Received: June 27, 2017      Accepted: November 20, Published: December 07, 2017 ABSTRACT Objective: aim this study was to investigate the effect mechanism by which progesterone regulates contraction in late pregnant rats Results: Progesterone caused concentration-dependent relaxation strips that enhanced compared with control nontreated strips. Uterine incubated showed a significant increase mRNA expression protein level. inhibitor L-methionine partly reversed progesterone, while activator arachidonic acid did not cause change progesterone-induced relaxation. Conclusions: inhibits induces pregnancy. inhibition appears involve increased potassium expression/activity. Materials Methods: Uterus from late-pregnant (gestational day 19) isolated, were prepared isometric measurement. Oxytocin-induced pretreated different concentration progesterone. agonist used determine whether changes activity. tissues measured using qPCR Western blot.