Long Noncoding RNA NEAT1 Upregulates Survivin and Facilitates Gallbladder Cancer Progression by Sponging microRNA-335.

摘要: Background Gallbladder cancer (GBC) is the most common of biliary tract, but molecularly targeted therapies are not available for GBC. Loss microRNA (miR)-335 expression may be a useful predictor clinical outcomes and reversal its loss treatment strategy In this study, we investigated whether long noncoding RNA, nuclear paraspeckle assembly transcript 1 (NEAT1) sponges miR-335 in GBC cells. Materials methods Quantitative reverse transcription-polymerase chain reaction (qRT-PCR), Western blotting, immunohistochemistry were used to determine miR-335; NEAT1; survivin; Ki67 cell lines (GBC-SD SGC-996) tissue samples from patients (n = 25). Cell Counting Kit-8, colony-formation, Transwell migration invasion assays performed measure proliferation, migration, invasion. Bioinformatic analysis dual-luciferase reporter utilized analyze correlativity. Results overexpression resulted inhibition proliferation addition, knockdown NEAT1 downregulation survivin expression. As competitively "sponges" miR-335, inhibited vitro tumor growth vivo. Furthermore, was found upregulated samples, inversely correlated with levels, positively levels. Conclusion These findings indicate that promotes by functioning as competitive endogenous RNA cells; thus, have identified potential biomarker target diagnosis therapy.