Primer pair, probe, kit and method for fluorescence quantitative PCR detection of FadV-4 type fowl adenoviruses

作者: Wei Xiaofeng , Hu Jianhua , Xiong Wei , Cai Xiaoyao , Zhang Quan

DOI:

关键词: DNAFluorescenceMolecular biologyPrimer (molecular biology)Specific detectionFowlNucleic acidReal-time polymerase chain reactionChemistry

摘要: The invention discloses a primer pair, probe, kit and method for fluorescence quantitative PCR detection of FadV-4 type fowl adenoviruses. takes sample DNA as template, SEQ ID No:1 No:2 primers No:3 the probe to perform amplification acquire signal. disclosed by can realize quick, accurate, convenient, rapid specific adenoviruse nucleic acids, is high in sensitivity, strong specificity good repeatability, has wide application prospects.

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