On the preparation of cryosections for immunocytochemistry

作者: Gareth Griffiths , Alasdair McDowall , Ruth Back , Jacques Dubochet

DOI: 10.1016/S0022-5320(84)80024-6

关键词: ImmunocytochemistryMethyl celluloseSucroseTissue cultureAnalytical chemistryLiquid nitrogenPelletsFrozen hydratedFrozen section procedureChemistryChromatographyAnatomyMolecular biology

摘要: The key preparation steps in the Tokuyasu thawed frozen section technique for immunocytochemistry, namely freezing, sectioning, thawing, and drying, were studied. A spherical …

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