The ras1 function of Schizosaccharomyces pombe mediates pheromone-induced transcription.

摘要: Loss of ras1+ function renders fission yeast cells unable to undergo morphological changes in response mating pheromones, whereas carrying activated mutations ras1 are hyper-responsive. This has led the suggestion that gene product plays a role pheromone signal transduction. Using partially purified M factor we demonstrate mat1-Pm gene, which controls entry into meiosis, is transcribed signal. Strains mutated or ste6, homologue Ras protein GDP/GTP exchange factor, induce transcription factor. Furthermore, an ras1val17 mutant exhibits stronger induction transcript. However, still depends on nitrogen deprivation as well presence pheromone, showing activation Ras1 alone does not substitute for any these signals. The pat1-114 bypasses ras1/ste6 checkpoint, suggesting contributes inactivation pat1 kinase.