Nonradioactive detection of telomerase activity using the telomeric repeat amplification protocol
作者： Brittney-Shea Herbert , Amelia E Hochreiter , Woodring E Wright , Jerry W Shay
关键词: Telomerase 、 Primer (molecular biology) 、 Fluorescence 、 Quantitative PCR analysis 、 Lysis 、 Tissue extracts 、 Molecular biology 、 Biology 、 Telomeric repeat
摘要: The telomeric repeat amplification protocol (TRAP) is a two-step process for analyzing telomerase activity in cell or tissue extracts. Recent modifications of this sensitive assay include elimination radioactivity by using fluorescently labeled primer instead radiolabeled primer. In addition, the TRAP has been modified real-time, quantitative PCR analysis. Here, we describe cost-effective procedures detection fluorescent-based as well real-time PCR. These assays can be accomplished within 4 h (from lysis samples to analysis products).
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