Effects of silymarin and silymarin-doxorubicin applications on telomerase activity of human hepatocellular carcinoma cell line HepG2.

摘要: PURPOSE Hepatocellular carcinoma (HCC) is resistant to conventional chemotherapeutics such as doxorubicin. Milk thistle extract, or its active constituent silymarin has been used by cancer patients an alternative and complementary agent. Telomerase activation one of the initial events HCC. In this study, we applied doxorubicin for 72 hrs in order test individual combined effect agents on telomerase activity. METHODS The effects doxorubicin, silymarin, their combination proliferation HepG2 cell line were tested MTT assay, Checkerboard micro plate method was define nature interactions cells. Lipid peroxidations assessed thiobarbituric acid reactive substance (TBARS) level. activity determined according telomeric repeat amplification protocol (TRAP). Untreated cells control group. RESULTS Doxorubicin-silymarin had indifferent antiproliferative incubated with IC50 decreased 72% (p<0.05). combinations caused 70% (p<0.05) reduction. All treatments except 1/2IC50 significant increase lipid peroxidation levels when compared controls. TBARS did not significantly combination, which concordance drug interaction. CONCLUSION both alone inhibited Mechanism inhibition may be elucidated further molecular studies.