Translocation by multi-subunit RNA polymerases

摘要: Abstract DNA template and RNA/DNA hybrid movement through RNA polymerase (RNAP) is referred to as “translocation”. Because nucleic acid coupled NTP loading, pyrophosphate release, conformational changes, the precise ordering of events during bond addition consequential. Moreover, based on several lines experimental evidence, translocation, release or an associated change may determine transcription elongation rate. In this review we discuss various models data supporting hypothesis that translocation rate determines also be inconsistent with point view. A model nucleotide cycle accommodating available proposed. On basis model, molecular mechanisms regulating potential routes for entry are discussed.