The Salmonella typhimurium his operon leader region contains an RNA hairpin-dependent transcription pause site. Mechanistic implications of the effect on pausing of altered RNA hairpins.
作者： C.L. Chan , R Landick
DOI: 10.1016/S0021-9258(19)47132-9
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摘要: The Salmonella typhimurium his leader region contains a well documented transcription attenuator. We report here the results of in vitro studies that characterized pause site region. occurred after synthesis first secondary structure (A:B) and immediately preceding addition G103 to nascent transcript. RNA polymerase pausing at this would allow ribosome synthesizing peptide release paused synchronize translation half-life complexes was enhanced by NusA, but not guanosine 5′-diphosphate 3′-diphosphate. Nuclease digestion modeling were consistent with compact three-dimensional for RNA. complex decreased greatly on altered templates which C71-G93 base pair disrupted unchanged when C65-G100 disrupted. This result is model portion RNA:DNA elongation heteroduplex retained.
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