Immunogenicity of living and heat-killed Salmonella pullorum vaccines.

摘要: Specific pathogen-free CD-1 and C57Bl mice were infected in a hind footpad with 5 x 10(4) viable Salmonella enteritidis cells or 10(7) S. pullorum cells. The resulting bacterial growth within the footpad, draining lymph nodes, liver spleen was followed for 14 days. Mice vaccinated live rapidly developed an effective antibacterial resistance to both intravenous intragastric challenge SM(R). inoculum of eliminated from normal mouse tissues failed induce detectable anti-Salmonella parenteral oral enteritidis. Heat-killed saline suspensions (200 mug, dry wt) unable antimicrobial against subsequent virulent challenge. However, when organisms suspended Freund complete adjuvant, vaccines induced Reduction antigenic dose 200 40 mug did not greatly affect protective value two killed challenge, but level protection observed 40-mug doses considerably reduced animals intragastrically, suggesting that some quantitative differences existed between sensitizing contents test organisms.