Vaccination of chickens with a Salmonella enteritidis aroA live oral Salmonella vaccine.

摘要: A mouse-virulent strain of Salmonella enteritidis, Se795 (LD50 less than 10 organisms for mice), was non-virulent 12-day-old chickens given 10(6) cfu intravenously; the were cleared from liver and spleen by day 14 as measured direct plating 21 enrichment. An Se795aroA mutant, CU58, also after intravenous inoculation 10(7) cfu. Day-old chicks vaccinated orally with either one dose 10(9) CU58 at 1 age, days, or 10(5) 7 days followed challenged a nalidixic acid resistant variant virulent phage type 4 S. enteritidis 109. All groups showed reduction in faecal shedding challenge. Chickens four doses significant liver, faeces following challenge Intramuscular vaccination Aro age gave no protection against oral Serum antibody production to LPS (ELISA) minimal all birds. The results indicate that Aro- can confer old enteritidis.