Endoplasmic reticulum (ER) stress and cAMP/PKA pathway mediated Zn-induced hepatic lipolysis

摘要: The present study was performed to determine the effect of Zn exposure influencing endoplasmic reticulum (ER) stress, explore underlying molecular mechanism Zn-induced hepatic lipolysis in a fish species significance for aquaculture, yellow catfish Pelteobagrus fulvidraco. We found that waterborne evoked ER stress and unfolded protein response (UPR), activated cAMP/PKA pathway, up-regulated lipolysis. increase were associated with activation signaling pathway. also induced an intracellular Ca2+ level, which could be partially prevented by dantrolene (RyR receptor inhibitor) 2-APB (IP3 inhibitor), demonstrating disturbed homeostasis contributed dysregulation Inhibition PBA attenuated UPR, inhibited pathway resulted down-regulation kinase RNA-activated-like (PERK) GSK2656157 inositol-requiring enzyme (IRE) STF-083010 differentially influenced changes lipid metabolism, indicating PERK IRE pathways played different regulatory roles PKA H89 blocked concomitant inhibition stress-mediated Taken together, our findings highlight importance stress-cAMP/PKA axis lipolysis, provides new insights into toxicology probably other vertebrates.