Cloning and Characterization of a cDNA That Encodes a 70-kDa Novel Human Thyroid Autoantigen
作者： J Y Chan , M I Lerman , B S Prabhakar , O Isozaki , P Santisteban
DOI: 10.1016/S0021-9258(19)84898-6
关键词: Open reading frame 、 Genomic library 、 Molecular biology 、 Nucleic acid sequence 、 Messenger RNA 、 Molecular cloning 、 Complementary DNA 、 Biology 、 Polyadenylation 、 Immunoprecipitation 、 Cell biology 、 Biochemistry
摘要: Abstract cDNA clones were isolated by screening a human thyroid carcinoma lambda gt11 library with immunoglobulins purified from serum of patient autoimmune Graves' disease. One clone (ML8) containing 1.25-kilobase (kb) insert hybridized single 2.0-kb poly(A+) mRNA in and lymphocytes but not brain, liver, kidney, or muscle. In addition, this probe also rat cell line (FRTL-5). An apparently full length 2,074-base pair (bp) was obtained sequenced. The nucleotide sequence the 2,074-bp includes 5'-noncoding 17 bp, 1827-bp open reading frame, 222-bp 3'-noncoding sequence. canonical polyadenylation signal AATAAA is present 18 bp upstream poly(A) tail. This encodes 69,812-dalton protein two potential N-linked glycosylation sites at least one membrane spanning domain. Immunoprecipitation vitro translated sera several patients disease argues that an autoantigen.
elsevier.com
sci-hub.st 参考文章(27)
Ira D. Goldfine, Syed M. Amir, Sidney H. Ingbar, Georgianne Tucker, The interaction of radioiodinated thyrotropin with plasma membranes Biochimica et Biophysica Acta (BBA) - Biomembranes. ,vol. 448, pp. 45- 56 ,(1976) , 10.1016/0005-2736(76)90075-4
Joachim Messing, New M13 vectors for cloning. Methods in Enzymology. ,vol. 101, pp. 20- 78 ,(1983) , 10.1016/0076-6879(83)01005-8
Tom A. Rapoport, GÜNter Blobel, Protein translocation across and integration into membranes. CRC critical reviews in biochemistry. ,vol. 20, pp. 73- 137 ,(1986) , 10.3109/10409238609115901
Jack Kyte, Russell F. Doolittle, A simple method for displaying the hydropathic character of a protein Journal of Molecular Biology. ,vol. 157, pp. 105- 132 ,(1982) , 10.1016/0022-2836(82)90515-0
W. J. Wilbur, D. J. Lipman, Rapid similarity searches of nucleic acid and protein data banks. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 80, pp. 726- 730 ,(1983) , 10.1073/PNAS.80.3.726
J. V. Maizel, R. P. Lenk, Enhanced graphic matrix analysis of nucleic acid and protein sequences. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 78, pp. 7665- 7669 ,(1981) , 10.1073/PNAS.78.12.7665
J.S. Baumstark, R.J. Laffin, W.A. Bardawil, A Preparative method for the separation of 7s gamma globulin from human serum Archives of Biochemistry and Biophysics. ,vol. 108, pp. 514- 522 ,(1964) , 10.1016/0003-9861(64)90436-9
FREDRIKA PEKONEN, BRUCE D. WEINTRAUB, Thyrotropin binding to cultured lymphocytes and thyroid cells. Endocrinology. ,vol. 103, pp. 1668- 1677 ,(1978) , 10.1210/ENDO-103-5-1668
PAOLO VITTI, CARLO M. ROTELLA, WILLIAM A. VALENTE, JOSHUA COHEN, SALVATORE M. ALOJ, PAOLO LACCETTI, FRANCESCO S. AMBESI-IMPIOMBATO, EVELYN F. GROLLMAN, ALDO PINCHERA, ROBERTO TOCCAFONDI, LEONARD D. KOHN, Characterization of the Optimal Stimulatory Effects of Graves’ Monoclonal and Serum Immunoglobulin G on Adenosine 3′,5′-Monophosphate Production in FRTL-5 Thyroid Cells: A Potential Clinical Assay The Journal of Clinical Endocrinology and Metabolism. ,vol. 57, pp. 782- 791 ,(1983) , 10.1210/JCEM-57-4-782
John Devereux, Paul Haeberli, Oliver Smithies, A comprehensive set of sequence analysis programs for the VAX Nucleic Acids Research. ,vol. 12, pp. 387- 395 ,(1984) , 10.1093/NAR/12.1PART1.387