Basal-like phenotype is not associated with patient survival in estrogen-receptor-negative breast cancers

摘要: Basal-phenotype or basal-like breast cancers are characterized by basal epithelium cytokeratin (CK5/14/17) expression, negative estrogen receptor (ER) status and distinct gene expression signature. We studied the clinical biological features of basal-phenotype tumors determined immunohistochemistry (IHC) cDNA microarrays especially within ER-negative subgroup. IHC was used to evaluate CK5/14 445 stage II cancers. The signature immunopositive investigated a subset (100) (including 50 tumors) with microarray. Survival for as assessed. From 375 analyzable tumor specimens, 48 (13%) were immunohistochemically positive CK5/14. found adverse distant disease-free survival CK5/14-positive during first years (3 hazard ratio (HR) 2.23, 95% confidence interval (CI) 1.17 4.24, p = 0.01; 5 HR 1.80, CI 1.02 3.15, 0.04) but significance lost at end follow-up period (10 1.43, 0.84 2.43, 0.19). Gene profiles group implicated 1,713 differently expressed genes (p < 0.05). Hierarchical clustering analysis top 500 these formed one non-basal-like cluster also entity. A highly concordant classification could be constructed published set (Sorlie's intrinsic set, concordance 90%). Both sets identified that included most tumors, CK5/14-negative tumors. Within entity there no difference between non-basal immunohistochemical gene-expression-based classification. Basal cytokeratin-positive have biologically from other Even if predicts early relapse among non-selected outcome is similar Immunohistochemically almost always belong profile, this includes few cytokeratin-negative