Structure and autonomic innervation of the swim bladder in the zebrafish (Danio rerio).

摘要: Many teleosts actively regulate buoyancy by using a gas-filled swim bladder, which is thought to be under autonomic control. Here we investigated the bladder in zebrafish determine possible mechanisms of gas-content regulation. Fluorescently labelled phalloidin revealed myocytes that appeared form sphincter at junction pneumatic duct and esophagus. Myocytes also formed thick bands along ventral surface anterior chamber bilaterally posterior chamber. Thinner layers were located elsewhere. Staining peroxidase within erythrocytes putative rete smaller blood vessels muscle The antibodies zn-12, general neuronal marker, SV2, synaptic vesicle marker labelling presynaptic terminals, widespread innervation system. Widespread was indicated acetylcholinesterase histochemistry, but choline acetyltransferase-immunoreactive (-IR) somata fibers limited In contrast, varicose tyrosine hydroxylase-IR innervated muscles throughout Neuropeptide Y-IR near esophagus vasculature duct. Vasoactive intestinal polypeptide immunoreactivity abundant sparsely distributed Serotonin-IR varicosities only Our results suggest complex richly organ buoyancy-regulating effectors may controlled multiple populations neurons.