Development of the swimbladder and its innervation in the zebrafish, Danio rerio.

摘要: Many teleosts including zebrafish, Danio rerio, actively regulate buoyancy with a gas-filled swimbladder, the volume of which is controlled by autonomic reflexes acting on vascular, muscular, and secretory effectors. In this study, we investigated morphological development zebrafish swimbladder together its effectors innervation. The first formed as single chamber, inflated at 1-3 days posthatching (dph), 3.5-4 mm body length. Lateral nerves were already present demonstrated antibody zn-12, blood vessels had in parallel cranial aspect to supply anastomotic capillary loops Tie-2 staining. Neuropeptide Y-(NPY-) like immunoreactive (LIR) fibers appeared early single-chambered stage, vasoactive intestinal polypeptide (VIP)-LIR cell bodies developed 10 dph (5 mm). By 18 (6 mm), anterior chamber evagination from end original chamber; both chambers then enlarged ductus communicans forming constriction between them. into an arteriovenous rete posterior region was innervated zn-12-reactive fibers. Tyrosine hydroxylase- (TH-), NPY-, VIP-LIR also area lateral chamber. Innervation 25-30 (8-9 band smooth muscle wall Although gas increased young larvae just after inflation, our results suggest that active control may not occur until formation two subsequent maturation vasculature, musculature innervation these structures about 28-30 dph.